BNIP1 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
BNIP1 polyclonal antibody (Cat # PAB7347) (0.5 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of BNIP1.
Immunogen
A synthetic peptide corresponding to human BNIP1.
Sequence
C-DLLRQRKTTKESLAQ
Host
Goat
Theoretical MW (kDa)
26.1
Reactivity
Human
Specificity
All reported variants represent isoform 1 (NP_001196.1, NP_053581.1, NP_053582.1, NP_053583.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:8000)
Western Blot (0.5-1.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
BNIP1 polyclonal antibody (Cat # PAB7347) (0.5 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — BNIP1
Entrez GeneID
662Protein Accession#
NP_001196.1;NP_053581.1;NP_053582.1;NP_053583.1Gene Name
BNIP1
Gene Alias
NIP1, SEC20, TRG-8
Gene Description
BCL2/adenovirus E1B 19kDa interacting protein 1
Omim ID
603291Gene Ontology
HyperlinkGene Summary
This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. Alternative splicing of this gene results in four protein products with identical N- and C-termini. [provided by RefSeq
Other Designations
BCL2/adenovirus E1B 19kD interacting protein 1|BCL2/adenovirus E1B 19kD-interacting protein 1|OTTHUMP00000161079
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Interactome
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Pathway
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Disease
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Publication Reference
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Involvement of BNIP1 in apoptosis and endoplasmic reticulum membrane fusion.
Nakajima K, Hirose H, Taniguchi M, Kurashina H, Arasaki K, Nagahama M, Tani K, Yamamoto A, Tagaya M.
The EMBO Journal 2004 Aug; 23(16):3216.
Application：IF, IP, WB-Tr, Human, HeLa, HEK 293T, MCF-7 cells.
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Involvement of BNIP1 in apoptosis and endoplasmic reticulum membrane fusion.
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