P4HA1 polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
P4HA1 polyclonal antibody (Cat # PAB7221) (7 ug/mL) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunofluorescence
P4HA1 polyclonal antibody (Cat # PAB7221) Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of P4HA1.
Immunogen
A synthetic peptide corresponding to human P4HA1.
Sequence
C-DFARKDEPDAFKE
Host
Goat
Theoretical MW (kDa)
61
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_000908.2 and NP_001017962.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Immunohistochemistry (7 ug/mL)
Imunofluorescence (10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
P4HA1 polyclonal antibody (Cat # PAB7221) (7 ug/mL) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.Immunofluorescence
P4HA1 polyclonal antibody (Cat # PAB7221) Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Enzyme-linked Immunoabsorbent Assay
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Gene Info — P4HA1
Entrez GeneID
5033Protein Accession#
NP_000908.2;NP_001017962.1Gene Name
P4HA1
Gene Alias
P4HA
Gene Description
prolyl 4-hydroxylase, alpha polypeptide I
Omim ID
176710Gene Ontology
HyperlinkGene Summary
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq
Other Designations
C-P4Halpha(I)|collagen prolyl 4-hydroxylase alpha(I)|procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I|prolyl 4-hydroxylase, alpha I subunit
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Interactome
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Pathway
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Disease
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Publication Reference
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Hypoxia potentiates microRNA-mediated gene silencing through post-translational modification of Argonaute2.
Wu C, So J, Davis-Dusenbery BN, Qi HH, Bloch DB, Shi Y, Lagna G, Hata A.
Molecular and Cellular Biology 2011 Dec; 31(23):4760.
Application：WB-Tr, Human, U-2 OS cells.
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Cloning of the human prolyl 4-hydroxylase alpha subunit isoform alpha(II) and characterization of the type II enzyme tetramer. The alpha(I) and alpha(II) subunits do not form a mixed alpha(I)alpha(II)beta2 tetramer.
Annunen P, Helaakoski T, Myllyharju J, Veijola J, Pihlajaniemi T, Kivirikko KI.
The Journal of Biological Chemistry 1997 Jul; 272(28):17342.
Application：WB-Ce, Human, HT-1080, WI-38 cells.
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Hypoxia potentiates microRNA-mediated gene silencing through post-translational modification of Argonaute2.
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