FEZ1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
FEZ1 polyclonal antibody (Cat # PAB7151) (0.03 ug/mL) staining of human brain (frontal cortex) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
FEZ1 polyclonal antibody (Cat # PAB7151) (5 ug/mL) staining of paraffin embedded Human Cerebellum. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of FEZ1.
Immunogen
A synthetic peptide corresponding to human FEZ1.
Sequence
C-QNKQKEQRELMKK
Host
Goat
Theoretical MW (kDa)
45.1
Reactivity
Human, Rat
Specificity
This antibody is expected to recognize isoform 1 (NP_005094.1) only.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:128000)
Immunohistochemistry (5-8 ug/mL)
Western Blot (0.01-0.03 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
FEZ1 polyclonal antibody (Cat # PAB7151) (0.03 ug/mL) staining of human brain (frontal cortex) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
FEZ1 polyclonal antibody (Cat # PAB7151) (5 ug/mL) staining of paraffin embedded Human Cerebellum. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — FEZ1
Entrez GeneID
9638Protein Accession#
NP_005094.1Gene Name
FEZ1
Gene Alias
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Gene Description
fasciculation and elongation protein zeta 1 (zygin I)
Omim ID
604825Gene Ontology
HyperlinkGene Summary
This gene is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles. Expression of this gene in C. elegans unc-76 mutants can restore to the mutants partial locomotion and axonal fasciculation, suggesting that it also functions in axonal outgrowth. The N-terminal half of the gene product is highly acidic. Alternatively spliced transcript variants encoding different isoforms of this protein have been described. [provided by RefSeq
Other Designations
zygin 1
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Interactome
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Disease
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Publication Reference
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Identification of FEZ1 as a protein that interacts with JC virus agnoprotein and microtubules: role of agnoprotein-induced dissociation of FEZ1 from microtubules in viral propagation.
Suzuki T, Okada Y, Semba S, Orba Y, Yamanouchi S, Endo S, Tanaka S, Fujita T, Kuroda S, Nagashima K, Sawa H.
The Journal of Biological Chemistry 2005 Jul; 280(26):24948.
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Identification of FEZ1 as a protein that interacts with JC virus agnoprotein and microtubules: role of agnoprotein-induced dissociation of FEZ1 from microtubules in viral propagation.
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