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Last updated: 2023/3/26
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AIF1 polyclonal antibody 

  • Catalog # : PAB6990
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of AIF1.
  • Immunogen:
  • A synthetic peptide corresponding to human AIF1.
  • Sequence:
  • C-NKQFLDDPKYSSDED
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 16.7
  • Reactivity:
  • Human
  • Specificity:
  • This antibody is expected to recognize isoform 3 (NP_001614.3) only.
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:128000)
    Flow Cytometry (10 ug/mL)
    Immunofluorescence (10 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (6 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • AIF1 polyclonal antibody (Cat # PAB6990, 2.5 ug/mL) staining of paraffin embedded mouse brain. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
  • Immunofluorescence
  • Immunofluorescence
  • AIF1 polyclonal antibody (Cat # PAB6990) Immunofluorescence analysis of paraformaldehyde fixed CaCo-2 cells immobilized on Shi-fix? coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
  • Immunofluorescence
  • Immunofluorescence
  • AIF1 polyclonal antibody (Cat # PAB6990) Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on Shi-fix coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
  • Enzyme-linked Immunoabsorbent Assay
  • Flow Cytometry
  • Flow Cytometry
  • AIF1 polyclonal antibody (Cat # PAB6990) Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
  • Application Image
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Enzyme-linked Immunoabsorbent Assay
  • Gene Information
  • Entrez GeneID:
  • 199
  • Gene Name:
  • AIF1
  • Gene Alias:
  • AIF-1,IBA1,IRT-1
  • Gene Description:
  • allograft inflammatory factor 1
  • Gene Summary:
  • This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000029354,OTTHUMP00000029356,interferon gamma responsive transcript,ionized calcium-binding adapter molecule
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