CD274 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CD274.
Immunogen
A synthetic peptide corresponding to C-terminus of human CD274.
Sequence
CKKQSDTHLEET
Host
Goat
Theoretical MW (kDa)
33.3
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western blot (0.3-1.0 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (4-6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
CD274 polyclonal antibody (Cat # PAB6732) staining (0.3 ug/mL) of human placenta lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
CD274 polyclonal antibody (Cat # PAB6732) (4 ug/mL) staining of paraffin embedded human placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CD274
Entrez GeneID
29126Protein Accession#
NP_054862.1Gene Name
CD274
Gene Alias
B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1, PDCD1LG1, PDL1
Gene Description
CD274 molecule
Omim ID
605402Gene Ontology
HyperlinkOther Designations
CD274 antigen|OTTHUMP00000021029|programmed cell death 1 ligand 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Costimulatory B7-H1 in renal cell carcinoma patients: Indicator of tumor aggressiveness and potential therapeutic target.
Thompson RH, Gillett MD, Cheville JC, Lohse CM, Dong H, Webster WS, Krejci KG, Lobo JR, Sengupta S, Chen L, Zincke H, Blute ML, Strome SE, Leibovich BC, Kwon ED.
PNAS 2004 Dec; 101(49):17174.
Application:IHC-Fr, Human, Human renal cell carcinoma.
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Costimulatory B7-H1 in renal cell carcinoma patients: Indicator of tumor aggressiveness and potential therapeutic target.
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