CSK polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
CSK polyclonal antibody (Cat # PAB6039) (1 ug/mL) staining of mouse spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot (Cell lysate)
CSK polyclonal antibody (Cat # PAB6039) staining (0.5 ug/mL) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
CSK polyclonal antibody (Cat # PAB6039) staining (0.1 ug/mL) of paraffin embedded human tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Strong staining of germinal centres. B) High magnification view of germinal centre cells.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CSK.
Immunogen
A synthetic peptide corresponding to human CSK.
Sequence
C-EQLEHIKTHELHL
Host
Goat
Theoretical MW (kDa)
50.7
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.5-2 ug/mL)
Immunohistochemistry (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
CSK polyclonal antibody (Cat # PAB6039) (1 ug/mL) staining of mouse spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Western Blot (Cell lysate)
CSK polyclonal antibody (Cat # PAB6039) staining (0.5 ug/mL) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
CSK polyclonal antibody (Cat # PAB6039) staining (0.1 ug/mL) of paraffin embedded human tonsil. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Strong staining of germinal centres. B) High magnification view of germinal centre cells.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CSK
Entrez GeneID
1445Protein Accession#
NP_004374.1Gene Name
CSK
Gene Alias
MGC117393
Gene Description
c-src tyrosine kinase
Omim ID
124095Gene Ontology
HyperlinkOther Designations
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Interactome
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Pathway
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Disease
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Publication Reference
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Two additional protein-tyrosine kinases expressed in human lung: fourth member of the fibroblast growth factor receptor family and an intracellular protein-tyrosine kinase.
Holtrich U, Brauninger A, Strebhardt K, Rubsamen-Waigmann H.
PNAS 1991 Dec; 88(23):10411.
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Two additional protein-tyrosine kinases expressed in human lung: fourth member of the fibroblast growth factor receptor family and an intracellular protein-tyrosine kinase.
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