ALDH1A1 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
ALDH1A1 polyclonal antibody (Cat # PAB6018) staining (0.03 ug/mL) of human liver lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot (Transfected lysate)
HEK293 overexpressing ALDH1A1 and probed with ALDH1A1 polyclonal antibody (Cat # PAB6018) (mock transfection in first lane), tested by Origene.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ALDH1A1.
Immunogen
A synthetic peptide corresponding to C-terminus of human ALDH1A1.
Sequence
C-EVKTVTVKISQKNS
Host
Goat
Theoretical MW (kDa)
54.9
Reactivity
Human, Mouse, Rat
Specificity
This antibody may cross-react with ALDH1A2 (GeneID 8854) with one residue difference from this design and with ALDH2 (GeneID 217) with two residues difference from this design.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Western Blot (0.03-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
ALDH1A1 polyclonal antibody (Cat # PAB6018) staining (0.03 ug/mL) of human liver lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing ALDH1A1 and probed with ALDH1A1 polyclonal antibody (Cat # PAB6018) (mock transfection in first lane), tested by Origene.Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — ALDH1A1
Entrez GeneID
216Protein Accession#
NP_000680.2Gene Name
ALDH1A1
Gene Alias
ALDC, ALDH-E1, ALDH1, ALDH11, MGC2318, PUMB1, RALDH1
Gene Description
aldehyde dehydrogenase 1 family, member A1
Gene Ontology
HyperlinkGene Summary
This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a cytosolic isoform, which has a high affinity for aldehydes. [provided by RefSeq
Other Designations
ALDH class 1|acetaldehyde dehydrogenase 1|aldehyde dehydrogenase 1, soluble|aldehyde dehydrogenase 1A1|aldehyde dehydrogenase, liver cytosolic|retinal dehydrogenase 1|retinaldehyde dehydrogenase 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Developmental expression of aldehyde dehydrogenase in rat: a comparison of liver and lung development.
Yoon M, Madden MC, Barton HA.
Toxicological Sciences 2005 Nov; 89(2):386.
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Retinoic acid imprints gut-homing specificity on T cells.
Iwata M, Hirakiyama A, Eshima Y, Kagechika H, Kato C, Song SY.
Immunity 2004 Oct; 21(4):527.
Application：IF, IHC-Fr, Mouse, Small intestine, Spleen.
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Developmental expression of aldehyde dehydrogenase in rat: a comparison of liver and lung development.
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