RB1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of RB1.
Immunogen
A synthetic peptide corresponding to residues surrounding S807 of human RB1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extract from MOLT 4 cells using RB1 polyclonal antibody (Cat # PAB5554) .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using RB1 polyclonal antibody (Cat # PAB5554) . -
Gene Info — RB1
Entrez GeneID
5925Gene Name
RB1
Gene Alias
OSRC, RB, p105-Rb, pRb, pp110
Gene Description
retinoblastoma 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq
Other Designations
OTTHUMP00000018397|retinoblastoma suspectibility protein|retinoblastoma-associated protein
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Interactome
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Pathway
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Disease
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Publication Reference
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Overexpression and hyperphosphorylation of retinoblastoma protein in the progression of malignant melanoma.
Roesch A, Becker B, Meyer S, Hafner C, Wild PJ, Landthaler M, Vogt T.
Modern Pathology: an Official Journal of the United States and Canadian Academy of Pathology, Inc 2005 Apr; 18(4):565.
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MLK3 is required for mitogen activation of B-Raf, ERK and cell proliferation.
Chadee DN, Kyriakis JM.
Nature Cell Biology 2004 Aug; 6(8):770.
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Dual mechanisms for the inhibition of E2F binding to RB by cyclin-dependent kinase-mediated RB phosphorylation.
Knudsen ES, Wang JY.
Molecular and Cellular Biology 1997 Oct; 17(10):5771.
Application:IP, IP-WB, WB-Tr, Human, BOSC293, C33-A cells.
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Overexpression and hyperphosphorylation of retinoblastoma protein in the progression of malignant melanoma.
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