PRKCB polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PRKCB.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PRKCB.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of PRKCB polyclonal antibody (Cat # PAB4532) in placenta lysate . PRKCB (arrow) was detected using purified PRKCB polyclonal antibody (Cat # PAB4532) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PRKCB polyclonal antibody (Cat # PAB4532) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRKCB
Entrez GeneID
5579Protein Accession#
P05771Gene Name
PRKCB
Gene Alias
MGC41878, PKC-beta, PKCB, PRKCB1, PRKCB2
Gene Description
protein kinase C, beta
Omim ID
176970Gene Ontology
HyperlinkGene Summary
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
protein kinase C, beta 1 polypeptide
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Interactome
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Pathway
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Disease
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Publication Reference
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Protein kinase C-beta-mediated complex formation between tyrosinase and TRP-1.
Wu H, Park HY.
Biochemical and Biophysical Research Communications 2003 Nov; 311(4):948.
Application:WB, Human, Human melanocytes.
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Centrosomal anchoring of protein kinase C betaII by pericentrin controls microtubule organization, spindle function, and cytokinesis.
Chen D, Purohit A, Halilovic E, Doxsey SJ, Newton AC.
The Journal of Biological Chemistry 2004 Feb; 279(6):4829.
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A chromatin immunoprecipitation screen reveals protein kinase Cbeta as a direct RUNX1 target gene.
Hug BA, Ahmed N, Robbins JA, Lazar MA.
The Journal of Biological Chemistry 2003 Oct; 279(2):825.
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Protein kinase C-beta-mediated complex formation between tyrosinase and TRP-1.
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