GFAP polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot analysis of GFAP (arrow) using GFAP polyclonal antibody (Cat # PAB3851).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GFAP gene (Lane 2).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with GFAP polyclonal antibody (Cat # PAB3851) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of GFAP.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human GFAP.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of GFAP (arrow) using GFAP polyclonal antibody (Cat # PAB3851).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GFAP gene (Lane 2).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with GFAP polyclonal antibody (Cat # PAB3851) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. -
Gene Info — GFAP
Entrez GeneID
2670Protein Accession#
NP_002046Gene Name
GFAP
Gene Alias
FLJ45472
Gene Description
glial fibrillary acidic protein
Gene Ontology
HyperlinkGene Summary
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq
Other Designations
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Interactome
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Disease
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Publication Reference
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Molecular genetic study in Japanese patients with Alexander disease: a novel mutation, R79L.
Shiroma N, Kanazawa N, Kato Z, Shimozawa N, Imamura A, Ito M, Ohtani K, Oka A, Wakabayashi K, Iai M, Sugai K, Sasaki M, Kaga M, Ohta T, Tsujino S.
Brain & Development 2003 Mar; 25(2):116.
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Detection of glial fibrillary acidic protein and neurofilaments in the cerebrospinal fluid of patients with neurocysticercosis.
Quintanar JL, Franco LM, Salinas E.
Parasitology Research 2003 Jul; 90(4):261.
Application：WB, Human, Cerebrospinal fluid of patients with neurocysticercosis.
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A new splice variant of glial fibrillary acidic protein, GFAP epsilon, interacts with the presenilin proteins.
Nielsen AL, Holm IE, Johansen M, Bonven B, Jorgensen P, Jorgensen AL.
The Journal of Biological Chemistry 2002 Aug; 277(33):29983.
Application：IF, WB-Tr, Human, HEK 293, SVG(P12) cells.
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Molecular genetic study in Japanese patients with Alexander disease: a novel mutation, R79L.
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