CD4 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of CD4 polyclonal antibody (Cat # PAB3534) in HeLa cell line lysates (35 ug/lane). CD4 (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).
Western Blot (Transfected lysate)
(RIGHT) Western blot analysis of CD4 (arrow) using rabbit CD4 polyclonal antibody (Cat # PAB3534). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD4 gene (Lane 2) (Origene Technologies).
Flow Cytometry
Flow cytometric analysis of CEM cells using CD4 polyclonal antibody (Cat # PAB3534)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CD4.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 58-86 at N-terminus of human CD4.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Flow Cytometry (1:10-50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CD4 polyclonal antibody (Cat # PAB3534) in HeLa cell line lysates (35 ug/lane). CD4 (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).Western Blot (Transfected lysate)
(RIGHT) Western blot analysis of CD4 (arrow) using rabbit CD4 polyclonal antibody (Cat # PAB3534). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD4 gene (Lane 2) (Origene Technologies).Flow Cytometry
Flow cytometric analysis of CEM cells using CD4 polyclonal antibody (Cat # PAB3534)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — CD4
Entrez GeneID
920Protein Accession#
NP_000607;P01730Gene Name
CD4
Gene Alias
CD4mut
Gene Description
CD4 molecule
Omim ID
186940Gene Ontology
HyperlinkOther Designations
CD4 antigen|CD4 antigen (p55)|CD4 receptor
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Interactome
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Pathway
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Disease
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Publication Reference
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Structural basis for the interaction between focal adhesion kinase and CD4.
Garron ML, Arthos J, Guichou JF, McNally J, Cicala C, Arold ST.
Journal of Molecular Biology 2008 Feb; 375(5):1320.
Application：IF, IP, WB, Human, Human T cells.
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Sequence variation occurs in CD4 epitopes during early HIV infection.
Rychert J, Saindon S, Placek S, Daskalakis D, Rosenberg E.
Journal of Acquired Immune Deficiency Syndromes 2007 Nov; 46(3):261.
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Structural basis for the interaction between focal adhesion kinase and CD4.
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