VARS polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of VARS polyclonal antibody (Cat # PAB3528) in K-562 cell line lysates (35 ug/lane).VARS (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocarcinomareacted with VARS polyclonal antibody (Cat # PAB3528), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of VARS.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human VARS.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:10-50)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of VARS polyclonal antibody (Cat # PAB3528) in K-562 cell line lysates (35 ug/lane).VARS (arrow) was detected using the purified polyclonal antibody (1:1000 dilution).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocarcinomareacted with VARS polyclonal antibody (Cat # PAB3528), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — VARS
Entrez GeneID
7407Protein Accession#
NP_006286;P26640Gene Name
VARS
Gene Alias
G7A, VARS1, VARS2
Gene Description
valyl-tRNA synthetase
Omim ID
192150Gene Ontology
HyperlinkGene Summary
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. The protein encoded by this gene belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex. [provided by RefSeq
Other Designations
valine tRNA ligase 1, cytoplasmic|valyl-tRNA synthetase 2
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Three-dimensional reconstruction of the valyl-tRNA synthetase/elongation factor-1H complex and localization of the delta subunit.
Jiang S, Wolfe CL, Warrington JA, Norcum MT.
FEBS Letters 2005 Nov; 579(27):6049.
-
Toward the full set of human mitochondrial aminoacyl-tRNA synthetases: characterization of AspRS and TyrRS.
Bonnefond L, Fender A, Rudinger-Thirion J, Giege R, Florentz C, Sissler M.
Biochemistry 2005 Mar; 44(12):4805.
-
Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse.
Xie T, Rowen L, Aguado B, Ahearn ME, Madan A, Qin S, Campbell RD, Hood L.
Genome Research 2003 Dec; 13(12):2621.
-
Three-dimensional reconstruction of the valyl-tRNA synthetase/elongation factor-1H complex and localization of the delta subunit.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com