SARS-CoV-2 Beta Variant S polyclonal antibody
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More Files
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Enzyme-linked Immunoabsorbent Assay
Titration curves of SARS-CoV-2 Variant B.1.351 S polyclonal antibody (Cat # PAB31800) with EC50 of 43.55 ng/mL. 96-well corning ELISA plate was coated with S (SARS-CoV-2 Variant B.1.351) RBD Recombinant Protein (Cat # P6703) at a concentration of 2 ug/mL.
Result Data
Pseudoviral particle (PP) infection assay challenged by SARS-CoV-2 Variant B.1.351 S polyclonal antibody (Cat # PAB31800).
HEK293-ACE2 cells infected with SARS-CoV-2 pseudoviral particles under various amounts of SARS-CoV-2 Variant B.1.351 S polyclonal antibody (Cat # PAB31800), with IC50 of 0.64 ug/mL (SARS-CoV-2 Pseudoviral Particles, D614G) and 1.17 ug/mL (SARS-CoV-2 Pseudoviral Particles, South Africa Variant).
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant SARS-CoV-2 Beta Variant S.
Immunogen
Recombinant protein corresponding to full length SARS-CoV-2 Variant B.1.351 (GISAID No. EPI_ISL_736980) S.
Host
Rabbit
Reactivity
SARS-CoV-2
Specificity
Reacts with spike protein of all SARS-CoV-2 variants tested. Cross-reaction to spike proteins from other coronavirus not tested.
Form
Liquid
Purification
Protein G immunoaffinity chromatography
Isotype
IgG
Recommend Usage
ELISA (1:40000)
Neutralization Assay
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS.
Storage Instruction
Store at 4°C is stable for 6 months from the date of shipment. For long term storage, store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
Result Data
Pseudoviral particle (PP) infection assay challenged by SARS-CoV-2 Variant B.1.351 S polyclonal antibody (Cat # PAB31800).
HEK293-ACE2 cells infected with SARS-CoV-2 pseudoviral particles under various amounts of SARS-CoV-2 Variant B.1.351 S polyclonal antibody (Cat # PAB31800), with IC50 of 0.64 ug/mL (SARS-CoV-2 Pseudoviral Particles, D614G) and 1.17 ug/mL (SARS-CoV-2 Pseudoviral Particles, South Africa Variant).
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Applications
Western Blot
Enzyme-linked Immunoabsorbent Assay
Titration curves of SARS-CoV-2 Variant B.1.351 S polyclonal antibody (Cat # PAB31800) with EC50 of 43.55 ng/mL. 96-well corning ELISA plate was coated with S (SARS-CoV-2 Variant B.1.351) RBD Recombinant Protein (Cat # P6703) at a concentration of 2 ug/mL.Neutralization
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