IFI16 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of HEK293T cell lysate using IFI16 polyclonal antibody (Cat # PAB28576).
Lane 1: Negative control (vector only)
Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human skin with IFI16 polyclonal antibody (Cat # PAB28576) shows strong nuclear positivity in epidermal cells. Retrieval method: HIER pH6
Immunofluorescence
Immunofluorescence of U-251 MG cell line with IFI16 polyclonal antibody (Cat # PAB28576) shows positivity in nucleus and nucleoli. Fixation/Permeabilization: PFA/Triton X-104
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant IFI16.
Immunogen
Recombinant protein corresponding to human IFI16.
Sequence
KEKFTPKKIIAIANYVCRNGFLEVYPFTLVADVNADRNMEIPKGLIRSASVTPKINQLCSQTKGSFVNGVFEVHKKNVRGEFTYYEIQDNTGKMEVVVHGRLTTINCEEGDKLKLTCFELAPKSGNTGELRSVIHSHIKVIK
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1:100-1:250)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Immunofluorescence (1-4 ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of HEK293T cell lysate using IFI16 polyclonal antibody (Cat # PAB28576).
Lane 1: Negative control (vector only)
Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human skin with IFI16 polyclonal antibody (Cat # PAB28576) shows strong nuclear positivity in epidermal cells. Retrieval method: HIER pH6Immunofluorescence
Immunofluorescence of U-251 MG cell line with IFI16 polyclonal antibody (Cat # PAB28576) shows positivity in nucleus and nucleoli. Fixation/Permeabilization: PFA/Triton X-104 -
Gene Info — IFI16
Entrez GeneID
3428Protein Accession#
E7EPR3Gene Name
IFI16
Gene Alias
IFNGIP1, MGC9466, PYHIN2
Gene Description
interferon, gamma-inducible protein 16
Omim ID
147586Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. [provided by RefSeq
Other Designations
OTTHUMP00000035192|interferon-gamma induced protein IFI 16|interferon-inducible myeloid differentiation transcriptional activator
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Interactome
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Disease
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