ACTN4 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with ACTN4 polyclonal antibody (Cat # PAB28526) at 1:100-1:500 dilution.
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with ACTN4 polyclonal antibody (Cat # PAB28526) at 1:100-1:250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human liver with ACTN4 polyclonal antibody (Cat # PAB28526) shows strong cytoplasmic and membranous positivity in lymphoid tissue in bile duct cells at 1:1000-1:2500 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line A-431 with ACTN4 polyclonal antibody (Cat # PAB28526) at 1-4 ug/ml shows positivity in nucleus.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant ACTN4.
Immunogen
Recombinant protein corresponding to amino acids of human ACTN4.
Sequence
MFIVHTIEEIEGLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTTVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANVVGPWIQTKMEEIGRISIEMNGTLE
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1:100-1:500)
Immunofluorescence (1-4 ug/ml)
Immunohistochemistry (1:1000-1:2500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with ACTN4 polyclonal antibody (Cat # PAB28526) at 1:100-1:500 dilution.Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with ACTN4 polyclonal antibody (Cat # PAB28526) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human liver with ACTN4 polyclonal antibody (Cat # PAB28526) shows strong cytoplasmic and membranous positivity in lymphoid tissue in bile duct cells at 1:1000-1:2500 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with ACTN4 polyclonal antibody (Cat # PAB28526) at 1-4 ug/ml shows positivity in nucleus. -
Gene Info — ACTN4
Entrez GeneID
81Protein Accession#
D6PXK4Gene Name
ACTN4
Gene Alias
ACTININ-4, DKFZp686K23158, FSGS, FSGS1
Gene Description
actinin, alpha 4
Gene Ontology
HyperlinkGene Summary
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq
Other Designations
actinin alpha4 isoform
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Interactome
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Pathway
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Disease
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