BRAF polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of BRAF.
Immunogen
A synthetic peptide corresponding to 19 amino acids at internal region of human BRAF.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
At least two isoforms of B-raf are known to exist; this antibody will detect both isoforms. This antibody will not cross-react with C-raf.
Form
Liquid
Purification
Peptide affinity purification
Concentration
1 mg/mL
Isotype
IgG
Recommend Usage
Western Blot (1-2 ug/mL)
Immunohistochemistry (2.5 ug/mL)
Immunofluorescence (20 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of BRAF in human brain tissue with BRAF polyclonal antibody (Cat # PAB27438) at (lane 1) 1 and (lane 2) 2 ug/mL.Immunohistochemistry
Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — BRAF
Entrez GeneID
673Protein Accession#
NP_004324Gene Name
BRAF
Gene Alias
B-RAF1, BRAF1, FLJ95109, MGC126806, MGC138284, RAFB1
Gene Description
v-raf murine sarcoma viral oncogene homolog B1
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq
Other Designations
94 kDa B-raf protein|B-Raf proto-oncogene serine/threonine-protein kinase (p94)|Murine sarcoma viral (v-raf) oncogene homolog B1
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Interactome
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