KLF4 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of KLF4.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human KLF4.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse stomach tissue lysate (35 ug/lane) with KLF4 polyclonal antibody (Cat # PAB2566).Western Blot (Cell lysate)
Western blot analysis of multiple cells cell lysate (35 ug/lane) with KLF4 polyclonal antibody (Cat # PAB2566).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with KLF4 polyclonal antibody (Cat # PAB2566) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human spleenreacted with KLF4 polyclonal antibody (Cat # PAB2566), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — KLF4
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Interactome
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Disease
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Publication Reference
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Kruppel-like factor 4 is essential for inflammatory monocyte differentiation in vivo.
Alder JK, Georgantas RW 3rd, Hildreth RL, Kaplan IM, Morisot S, Yu X, McDevitt M, Civin CI.
Journal of Immunology 2008 Apr; 180(8):5645.
Application:WB, Mouse, Mouse bone marrow, monocyte, spleen, thymus.
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Regulation of Kruppel-like factor 4, 9, and 13 genes and the steroidogenic genes LDLR, StAR, and CYP11A in ovarian granulosa cells.
Natesampillai S, Kerkvliet J, Leung PC, Veldhuis JD.
American Journal of Physiology. Endocrinology and Metabolism 2008 Feb; 294(2):E385.
Application:WB-Ce, Human, Granulosa-luteal and granulosa-cell extracts.
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Kruppel-like factor 4 is acetylated by p300 and regulates gene transcription via modulation of histone acetylation.
Evans PM, Zhang W, Chen X, Yang J, Bhakat KK, Liu C.
The Journal of Biological Chemistry 2007 Nov; 282(47):33994.
Application:IP-WB, Human, HCT116 cells.
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Kruppel-like factor 4 is essential for inflammatory monocyte differentiation in vivo.
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