EPHB6 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of EPHB6 polyclonal antibody (Cat # PAB2074) in A-549 cell lysate. EPHB6 (arrow) was detected using purified Polyclonal antibody.
Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with EPHB6 polyclonal antibody (Cat # PAB2074) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
Flow Cytometry
Flow cytometric analysis of MCF-7 cells using EPHB6 polyclonal antibody (Cat # PAB2074)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EPHB6.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human EPHB6.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of EPHB6 polyclonal antibody (Cat # PAB2074) in A-549 cell lysate. EPHB6 (arrow) was detected using purified Polyclonal antibody.
Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with EPHB6 polyclonal antibody (Cat # PAB2074) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.Flow Cytometry
Flow cytometric analysis of MCF-7 cells using EPHB6 polyclonal antibody (Cat # PAB2074)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — EPHB6
Entrez GeneID
2051Protein Accession#
NP_004436;O15197Gene Name
EPHB6
Gene Alias
HEP, MGC129910, MGC129911
Gene Description
EPH receptor B6
Omim ID
602757Gene Ontology
HyperlinkGene Summary
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ephrin receptor encoded by this gene lacks the kinase activity of most receptor tyrosine kinases and binds to ephrin-B ligands. [provided by RefSeq
Other Designations
ephrin receptor EphB6
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Interactome
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Pathway
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Publication Reference
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The EphB6 receptor inhibits JNK activation in T lymphocytes and modulates T cell receptor-mediated responses.
Freywald A, Sharfe N, Rashotte C, Grunberger T, Roifman CM.
The Journal of Biological Chemistry 2003 Mar; 278(12):10150.
Application：WB-Tr, Human, Jurkat cells.
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EphB6 crosslinking results in costimulation of T cells.
Luo H, Yu G, Wu Y, Wu J.
The Journal of Clinical Investigation 2002 Oct; 110(8):1141.
Application：Flow Cyt, IF, Human, Human T cells.
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Multiple roles of EPH receptors and ephrins in neural development.
Wilkinson DG.
Nature Reviews. Neuroscience 2001 Mar; 2(3):155.
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The EphB6 receptor inhibits JNK activation in T lymphocytes and modulates T cell receptor-mediated responses.
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