TGFBI polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of TGFBI.
Immunogen
A synthetic peptide corresponding to internal region of human TGFBI.
Sequence
C-QLYTDRTEKLRPE
Host
Goat
Theoretical MW (kDa)
70
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (0.2-0.5 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (4-6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
TGFBI polyclonal antibody (Cat # PAB18750) (0.3 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
TGFBI polyclonal antibody (Cat # PAB18750) (3.8 ug/mL) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — TGFBI
Entrez GeneID
7045Protein Accession#
NP_000349.1Gene Name
TGFBI
Gene Alias
BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, LCD1
Gene Description
transforming growth factor, beta-induced, 68kDa
Gene Ontology
HyperlinkGene Summary
This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq
Other Designations
RGD-containing collagen-associated protein|kerato-epithelin
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Interactome
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Disease
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