P2RX4 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
P2RX4 polyclonal antibody (Cat # PAB18695) (0.3 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Flow Cytometry
P2RX4 polyclonal antibody (Cat # PAB18695) Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of P2RX4.
Immunogen
A synthetic peptide corresponding to C-terminus of human P2RX4.
Sequence
C-YREKKYKYVEDYEQ
Host
Goat
Theoretical MW (kDa)
43.4
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:128000)
Flow Cytometry (10 ug/mL)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
P2RX4 polyclonal antibody (Cat # PAB18695) (0.3 ug/mL) staining of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
P2RX4 polyclonal antibody (Cat # PAB18695) Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — P2RX4
Entrez GeneID
5025Protein Accession#
NP_002551.2Gene Name
P2RX4
Gene Alias
P2X4, P2X4R
Gene Description
purinergic receptor P2X, ligand-gated ion channel, 4
Omim ID
600846Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel with high calcium permeability. The main pharmacological distinction between the members of the purinoceptor family is the relative sensitivity to the antagonists suramin and PPADS. The product of this gene has the lowest sensitivity for these antagonists. Multiple alternatively spliced transcript variants have been identified for this gene although their full-length natures have not been determined. [provided by RefSeq
Other Designations
ATP receptor|ATP-gated cation channel protein|P2X purinoceptor 4|P2X receptor, subunit 4|purinergic receptor P2X4|purinoceptor P2X4
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Interactome
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Pathway
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Disease
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