AIM2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
AIM2 polyclonal antibody (Cat # PAB18597) (1 ug/mL) staining of IFNbeta-treated WI-38 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
AIM2 polyclonal antibody (Cat # PAB18597) (3.8 ug/mL) staining of paraffin embedded human spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of AIM2.
Immunogen
A synthetic peptide corresponding to C-terminus of human AIM2.
Sequence
C-GEKLQLTSGVHSTIK
Host
Goat
Theoretical MW (kDa)
39
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (1-3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (4-6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
AIM2 polyclonal antibody (Cat # PAB18597) (1 ug/mL) staining of IFNbeta-treated WI-38 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
AIM2 polyclonal antibody (Cat # PAB18597) (3.8 ug/mL) staining of paraffin embedded human spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — AIM2
Entrez GeneID
9447Protein Accession#
NP_004824.1Gene Name
AIM2
Gene Alias
PYHIN4
Gene Description
absent in melanoma 2
Omim ID
604578Gene Ontology
HyperlinkGene Summary
AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2. [provided by RefSeq
Other Designations
OTTHUMP00000035296
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Interactome
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Disease
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