AOF2 polyclonal antibody
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More Files
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Western Blot (Transfected lysate)
Western blot analysis of AOF2 (arrow) using AOF2 polyclonal antibody (Cat # PAB1844). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AOF2 gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with AOF2 polyclonal antibody (Cat # PAB1844), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Aof 2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human AOF2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of AOF2 (arrow) using AOF2 polyclonal antibody (Cat # PAB1844). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AOF2 gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with AOF2 polyclonal antibody (Cat # PAB1844), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — AOF2
Entrez GeneID
23028Protein Accession#
NP_055828;O60341Gene Name
AOF2
Gene Alias
BHC110, KDM1, KIAA0601, LSD1
Gene Description
amine oxidase (flavin containing) domain 2
Omim ID
609132Gene Ontology
HyperlinkGene Summary
This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
BRAF35-HDAC complex protein BHC110|FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit|lysine (K)-specific demethylase 1|lysine-specific histone demethylase 1
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Interactome
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Publication Reference
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Histone demethylation catalysed by LSD1 is a flavin-dependent oxidative process.
Forneris F, Binda C, Vanoni MA, Mattevi A, Battaglioli E.
FEBS Letters 2005 Apr; 579(10):2203.
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Histone demethylation mediated by the nuclear amine oxidase homolog LSD1.
Shi Y, Lan F, Matson C, Mulligan P, Whetstine JR, Cole PA, Casero RA, Shi Y.
Cell 2004 Dec; 119(7):941.
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A candidate X-linked mental retardation gene is a component of a new family of histone deacetylase-containing complexes.
Hakimi MA, Dong Y, Lane WS, Speicher DW, Shiekhattar R.
The Journal of Biological Chemistry 2003 Feb; 278(9):7234.
Application：Func, WB, Human, HeLa cells.
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Histone demethylation catalysed by LSD1 is a flavin-dependent oxidative process.
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