CBL polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells, treated with EGF (200 ng/mL, 30 mins), using CBL polyclonal antibody (Cat # PAB18165).
Peptide "+" means "peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CBL polyclonal antibody (Cat # PAB18165).
Peptide "+" means "peptide blocking".
Immunofluorescence
Immunofluorescence analysis of A-549 cells, using CBL polyclonal antibody (Cat # PAB18165).
Peptide "+" means "peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CBL.
Immunogen
A synthetic peptide corresponding to human CBL.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to CBL.
Form
Liquid
Purification
Affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells, treated with EGF (200 ng/mL, 30 mins), using CBL polyclonal antibody (Cat # PAB18165).
Peptide "+" means "peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CBL polyclonal antibody (Cat # PAB18165).
Peptide "+" means "peptide blocking".Immunofluorescence
Immunofluorescence analysis of A-549 cells, using CBL polyclonal antibody (Cat # PAB18165).
Peptide "+" means "peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — CBL
Entrez GeneID
867Protein Accession#
P22681Gene Name
CBL
Gene Alias
C-CBL, CBL2, RNF55
Gene Description
Cas-Br-M (murine) ecotropic retroviral transforming sequence
Omim ID
165360Gene Ontology
HyperlinkGene Summary
The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation. [provided by RefSeq
Other Designations
oncogene CBL2
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Interactome
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Pathway
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Disease
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Publication Reference
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Spontaneous tumor rejection by cbl-b-deficient CD8+ T cells.
Loeser S, Loser K, Bijker MS, Rangachari M, van der Burg SH, Wada T, Beissert S, Melief CJ, Penninger JM.
The Journal of Experimental Medicine 2007 Apr; 204(4):879.
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Regulatory defects in Cbl and mitogen-activated protein kinase (extracellular signal-related kinase) pathways cause persistent hyperexpression of CD40 ligand in human lupus T cells.
Yi Y, McNerney M, Datta SK.
The Journal of Immunology 2000 Dec; 165(11):6627.
Application：WB-Ce, Human, Lupus T cells.
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Spontaneous tumor rejection by cbl-b-deficient CD8+ T cells.
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