APAF1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of APAF1.
Immunogen
A synthetic peptide corresponding to human APAF1.
Host
Rabbit
Reactivity
Human
Specificity
This antibody is specific to APAF1.
Form
Liquid
Recommend Usage
Western Blot (1:500~1:1000)
Immunohistochemistry (1:50~1:100)
ELISA(1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COLO 205, using APAF1 polyclonal antibody (Cat # PAB18086).
Peptide "+" means "with peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using APAF1 polyclonal antibody (Cat # PAB18086).
Peptide "+" means "with peptide blocking".Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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Gene Info — APAF1
Entrez GeneID
317Protein Accession#
O14727Gene Name
APAF1
Gene Alias
CED4, DKFZp781B1145
Gene Description
apoptotic peptidase activating factor 1
Omim ID
602233Gene Ontology
HyperlinkGene Summary
This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
apoptotic protease activating factor 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Oncogenic serine-threonine kinase receptor-associated protein modulates the function of Ewing sarcoma protein through a novel mechanism.
Anumanthan G, Halder SK, Friedman DB, Datta PK.
Cancer Research 2006 Nov; 66(22):10824.
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Cryptic promoter activity in the DNA sequence corresponding to the pim-1 5'-UTR.
Wang Z, Weaver M, Magnuson NS.
Nucleic Acids Research 2005 Apr; 33(7):2248.
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Inhibition of cytochrome c release in Fas-mediated signaling pathway in transgenic mice induced to express hepatitis C viral proteins.
Machida K, Tsukiyama-Kohara K, Seike E, Tone S, Shibasaki F, Shimizu M, Takahashi H, Hayashi Y, Funata N, Taya C, Yonekawa H, Kohara M.
The Journal of Biological Chemistry 2001 Apr; 276(15):12140.
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Oncogenic serine-threonine kinase receptor-associated protein modulates the function of Ewing sarcoma protein through a novel mechanism.
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