ADD1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells treated with Forskolin (40 nM, 30 min), using ADD1 polyclonal antibody (Cat # PAB18057).
Peptide "+" means "with peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using ADD1 polyclonal antibody (Cat # PAB18057).
Peptide "+" means "with peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ADD1.
Immunogen
A synthetic peptide corresponding to residues surrounding S726 of human ADD1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody is specific to ADD1.
Form
Liquid
Recommend Usage
Western Blot (1:500~1:1000)
Immunohistochemistry (1:50~1:100)
Immunofluorescence (1:500~1:1000)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells treated with Forskolin (40 nM, 30 min), using ADD1 polyclonal antibody (Cat # PAB18057).
Peptide "+" means "with peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using ADD1 polyclonal antibody (Cat # PAB18057).
Peptide "+" means "with peptide blocking".Immunohistochemistry
Immunofluorescence
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — ADD1
Entrez GeneID
118Protein Accession#
P35611Gene Name
ADD1
Gene Alias
ADDA, MGC3339, MGC44427
Gene Description
adducin 1 (alpha)
Gene Ontology
HyperlinkGene Summary
Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq
Other Designations
OTTHUMP00000151164|OTTHUMP00000151165|erythrocyte adducin alpha subunit
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Interactome
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Disease
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Publication Reference
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Chromatin remodeling complex interacts with ADD1/SREBP1c to mediate insulin-dependent regulation of gene expression.
Lee YS, Sohn DH, Han D, Lee HW, Seong RH, Kim JB.
Molecular and Cellular Biology 2006 Oct; 27(2):438.
Application：IP-WB, Human, Mouse, HeLa, NIH/3T3 cells, Mouse epididymal fat tissues.
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Regulatory role of glycogen synthase kinase 3 for transcriptional activity of ADD1/SREBP1c.
Kim KH, Song MJ, Yoo EJ, Choe SS, Park SD, Kim JB.
The Journal of Biological Chemistry 2004 Dec; 279(50):51999.
Application：IP, Human, HEK 293 cells.
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ADD1/SREBP-1c is required in the activation of hepatic lipogenic gene expression by glucose.
Foretz M, Pacot C, Dugail I, Lemarchand P, Guichard C, Le Liepvre X, Berthelier-Lubrano C, Spiegelman B, Kim JB, Ferre P, Foufelle F.
Molecular and Cellular Biology 1999 May; 19(5):3760.
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Chromatin remodeling complex interacts with ADD1/SREBP1c to mediate insulin-dependent regulation of gene expression.
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