ABL1 (phospho Y412) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from COS-7 cells treated with Adriamycin.
Lane 1 : Using ABL1 (phospho Y412) polyclonal antibody (Cat # PAB16947).
Lane 2 : Using the same antibody preincubated with synthesized peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ABL1 (phospho Y412) polyclonal antibody (Cat # PAB16947).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of ABL1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Y412 of human ABL1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of ABL1 only when phosphorylated at tyrosine 412.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:4000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM PBS, 0.15 M NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COS-7 cells treated with Adriamycin.
Lane 1 : Using ABL1 (phospho Y412) polyclonal antibody (Cat # PAB16947).
Lane 2 : Using the same antibody preincubated with synthesized peptide.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ABL1 (phospho Y412) polyclonal antibody (Cat # PAB16947).Enzyme-linked Immunoabsorbent Assay
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Gene Info — ABL1
Entrez GeneID
25Protein Accession#
P00519Gene Name
ABL1
Gene Alias
ABL, JTK7, bcr/abl, c-ABL, p150, v-abl
Gene Description
c-abl oncogene 1, receptor tyrosine kinase
Omim ID
189980Gene Ontology
HyperlinkGene Summary
The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1. The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript, with alternatively spliced first exons spliced to the common exons 2-11. [provided by RefSeq
Other Designations
Abelson murine leukemia viral (v-abl) oncogene homolog 1|OTTHUMP00000022375|OTTHUMP00000022376|bcr/c-abl oncogene protein|proto-oncogene tyrosine-protein kinase ABL1|v-abl Abelson murine leukemia viral oncogene homolog 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer.
Rikova K, Guo A, Zeng Q, Possemato A, Yu J, Haack H, Nardone J, Lee K, Reeves C, Li Y, Hu Y, Tan Z, Stokes M, Sullivan L, Mitchell J, Wetzel R, Macneill J, Ren JM, Yuan J, Bakalarski CE, Villen J, Kornhauser JM, Smith B, Li D, Zhou X, Gygi SP, Gu TL, Polakiewicz RD, Rush J, Comb MJ.
Cell 2007 Dec; 131(6):1190.
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Autoinhibition of c-Abl.
Pluk H, Dorey K, Superti-Furga G.
Cell 2002 Jan; 108(2):247.
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JAK-STAT signaling activated by Abl oncogenes.
Danial NN, Rothman P.
Oncogene 2000 May; 19(21):2523.
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Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer.
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