AAK1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of AAK1 in A-20 cell lysate with AAK1 polyclonal antibody (Cat # PAB16581) at (A) 1 and (B) 2 ug/mL .
Immunocytochemistry
Immunocytochemistry of AAK1 in A-20 cells with AAK1 polyclonal antibody (Cat # PAB16581) at 5 ug/mL .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of AAK1.
Immunogen
A synthetic peptide corresponding to N-terminus 20 amino acids of human AAK1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Recommend Usage
Western Blot (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of AAK1 in A-20 cell lysate with AAK1 polyclonal antibody (Cat # PAB16581) at (A) 1 and (B) 2 ug/mL .Immunocytochemistry
Immunocytochemistry of AAK1 in A-20 cells with AAK1 polyclonal antibody (Cat # PAB16581) at 5 ug/mL .Enzyme-linked Immunoabsorbent Assay
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Gene Info — AAK1
Entrez GeneID
22848Protein Accession#
NP_055726Gene Name
AAK1
Gene Alias
DKFZp686F03202, DKFZp686K16132, FLJ23712, FLJ25931, FLJ31060, FLJ42882, FLJ45252, KIAA1048, MGC138170, MGC164568, MGC164570
Gene Description
AP2 associated kinase 1
Gene Ontology
HyperlinkGene Summary
Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq
Other Designations
adaptor-associated kinase 1
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Interactome
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Disease
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Publication Reference
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A novel AAK1 splice variant functions at multiple steps of the endocytic pathway.
Henderson DM, Conner SD.
Molecular Biology of the Cell 2007 May; 18(7):2698.
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The Ark1/Prk1 family of protein kinases. Regulators of endocytosis and the actin skeleton.
Smythe E, Ayscough KR.
EMBO Reports 2003 Mar; 4(3):246.
Application：WB, Human, Mammalian cells.
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Identification of an adaptor-associated kinase, AAK1, as a regulator of clathrin-mediated endocytosis.
Conner SD, Schmid SL.
The Journal of Cell Biology 2002 Mar; 156(5):921.
Application：IF, WB, Human, Rat, HeLa cells, Rat hippocampal neurons.
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A novel AAK1 splice variant functions at multiple steps of the endocytic pathway.
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