NES polyclonal antibody (DyLight 650)
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant NES.
Immunogen
Recombinant protein corresponding to amino acids 1464-1614 of human NES.
Host
Rabbit
Reactivity
Human
Specificity
It does not cross-react with rodent protein. This is specific to human nestin. This antibody may be used in Immunocytochemistry/Immunofluorescence and Western blot where a band is seen at ~220-240 kDa. Another band may be seen ~110-115 kDa. Other applications have not been tested. Though this production has not been tested, past productions have been useful for Immunohistochemistry and Immunoprecipitation. The investigator should determine the optimal working dilution for a specific application.
Form
Liquid
Conjugation
DyLight 650
Purification
Immunogen affinity purification
Recommend Usage
Immunocytochemistry/Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM sodium borate (0.05% sodium azide).
Storage Instruction
Store at 4°C in the dark.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunocytochemistry
Immunofluorescence
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Gene Info — NES
Entrez GeneID
10763Protein Accession#
P48681Gene Name
NES
Gene Alias
FLJ21841, Nbla00170
Gene Description
nestin
Omim ID
600915Gene Ontology
HyperlinkGene Summary
Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM
Other Designations
OTTHUMP00000032198
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