ACP1 polyclonal antibody
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More Files
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Western Blot
CHO cells were transfected with either Vector (pSRa) or expressionvector driving expression of ACP1 (pSRa-LMWPTP), and harvested in Triton X-100 lysis buffer at 72 h after transfection. 2 mg of lysate protein was incubated for 1 h with ACP1 polyclonal antibody (Cat # PAB14684), and immuno-precipitated proteins recovered by Protein A/G were separated on PAGE (12%), and ACP1 detected using a mouse anti-sheep monoclonal antibody.
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Specification
Product Description
Sheep polyclonal antibody raised against partial recombinant ACP1.
Immunogen
Recombinant His fusion protein corresponding to human ACP1.
Host
Sheep
Reactivity
Human
Form
Liquid
Recommend Usage
Immunoprecipitation (1 to 10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.08% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
CHO cells were transfected with either Vector (pSRa) or expressionvector driving expression of ACP1 (pSRa-LMWPTP), and harvested in Triton X-100 lysis buffer at 72 h after transfection. 2 mg of lysate protein was incubated for 1 h with ACP1 polyclonal antibody (Cat # PAB14684), and immuno-precipitated proteins recovered by Protein A/G were separated on PAGE (12%), and ACP1 detected using a mouse anti-sheep monoclonal antibody. -
Gene Info — ACP1
Entrez GeneID
52Gene Name
ACP1
Gene Alias
HAAP, MGC111030, MGC3499
Gene Description
acid phosphatase 1, soluble
Omim ID
171500Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the phosphotyrosine protein phosphatase family of proteins. It functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. This enzyme also hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. This gene is genetically polymorphic, and three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
Other Designations
acid phosphatase of erythrocyte|adipocyte acid phosphatase|cytoplasmic phosphotyrosyl protein phosphatase|low molecular weight phosphotyrosine protein phosphatase|protein tyrosine phosphatase|red cell acid phosphatase 1
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Interactome
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Pathway
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Disease
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Publication Reference
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HCPTPA, a protein tyrosine phosphatase that regulates vascular endothelial growth factor receptor-mediated signal transduction and biological activity.
Huang L, Sankar S, Lin C, Kontos CD, Schroff AD, Cha EH, Feng SM, Li SF, Yu Z, Van Etten RL, Blanar MA, Peters KG.
The Journal of Biological Chemistry 1999 Dec; 274(53):38183.
Application：WB-Tr, Human, HUVECs.
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Surface densities of ephrin-B1 determine EphB1-coupled activation of cell attachment through alphavbeta3 and alpha5beta1 integrins.
Huynh-Do U, Stein E, Lane AA, Liu H, Cerretti DP, Daniel TO.
The EMBO Journal 1999 Apr; 18(8):2165.
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Eph receptors discriminate specific ligand oligomers to determine alternative signaling complexes, attachment, and assembly responses.
Stein E, Lane AA, Cerretti DP, Schoecklmann HO, Schroff AD, Van Etten RL, Daniel TO.
Genes & Development 1998 Mar; 12(5):667.
Application：WB-Ce, Human, Mouse, Human renal microvascular endothelial cells, P19 cells.
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HCPTPA, a protein tyrosine phosphatase that regulates vascular endothelial growth factor receptor-mediated signal transduction and biological activity.
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