BDNF polyclonal antibody
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More Files
- More Functions
Western Blot
Western Blot analysis of (1) BDNF of rhBDNF R-088-100 (whole serum), (2) BDNF-isoform of rhBDNF R-088-100 (whole serum), (3) SHSY5Y of rhBDNF R-088-100 (whole serum), (4) Human brain of rhBDNF R-088-100 (whole serum), (5) Human brain of R-017-500 (IgG, 10 ug/mL), monomeric BDNF at 14 kDa and proBDNF is detected at the expected molecular weight of 32 kDa for glycosylated proBDNF monomer.
Immunohistochemistry
Immunohistochemical staining for BDNF in rat dorsal root ganglion (DRG) using BDNF polyclonal antibody (Cat # PAB14311) at an amount of 5 ug/mL . Neuron cell bodies are strongly stained for BDNF.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant BDNF.
Immunogen
Recombinant protein corresponding to human BDNF.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody has less than 0.1% cross-reactivity against NGF, recombinant NT3 and NT4 by dot blot.
Form
Lyophilized
Purification
Protein G purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1-10 ug/mL)
ELISA (0.1 ug/mL)
Inhibition Assay (1-10 ug/mL)
Western Blot (1-10 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Reconstitute in 500 uL sterile-filtered 1X PBS, pH 7.2-7.6. Glycerol (1:1) may be added for an additional stability.
Storage Instruction
Store at 4°C on dry atmosphere. After reconstitution store at -20°C
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western Blot analysis of (1) BDNF of rhBDNF R-088-100 (whole serum), (2) BDNF-isoform of rhBDNF R-088-100 (whole serum), (3) SHSY5Y of rhBDNF R-088-100 (whole serum), (4) Human brain of rhBDNF R-088-100 (whole serum), (5) Human brain of R-017-500 (IgG, 10 ug/mL), monomeric BDNF at 14 kDa and proBDNF is detected at the expected molecular weight of 32 kDa for glycosylated proBDNF monomer.Immunohistochemistry
Immunohistochemical staining for BDNF in rat dorsal root ganglion (DRG) using BDNF polyclonal antibody (Cat # PAB14311) at an amount of 5 ug/mL . Neuron cell bodies are strongly stained for BDNF.Enzyme-linked Immunoabsorbent Assay
Inhibition Assay
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Gene Info — BDNF
Entrez GeneID
627Gene Name
BDNF
Gene Alias
MGC34632
Gene Description
brain-derived neurotrophic factor
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq
Other Designations
neurotrophin
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Interactome
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Pathway
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Disease
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Publication Reference
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Physiology of BDNF: focus on hypothalamic function.
Tapia-Arancibia L, Rage F, Givalois L, Arancibia S.
Frontiers in Neuroendocrinology 2004 Jul; 25(2):77.
Application：IHC, Rat, Rat brain.
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The BDNF gene: exemplifying complexity in Ca2+ -dependent gene expression.
Mellstrom B, Torres B, Link WA, Naranjo JR.
Crit Rev Neurobiol 2004 Jan; 16(1-2):43.
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BDNF: a neuromodulator in nociceptive pathways?
Pezet S, Malcangio M, McMahon SB.
Brain Research. Brain Research Reviews 2002 Oct; 40(1-3):240.
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Physiology of BDNF: focus on hypothalamic function.
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