CAMK2A polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
CAMK2A polyclonal antibody (Cat # PAB14145) (0.1 ug/mL) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
CAMK2A polyclonal antibody (Cat # PAB14145) (3.8 ug/mL) staining of paraffin embedded human brain cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CAMK2A.
Immunogen
A synthetic peptide corresponding to internal region of human CAMK2A.
Sequence
C-PRTAQSEETRVWHR
Host
Goat
Theoretical MW (kDa)
55.3, 54.1
Reactivity
Human, Mouse
Specificity
Approx 55 and 65 KDa bands observed in human brain (cerebellum) and mouse brain lysates (calculated MW of 55.3 KDa according to human NP_057065.2 and of 54.1 KDa according to human NP_741960.1 and mouse NP_803126.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:64000)
Western Blot (0.1-0.3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
CAMK2A polyclonal antibody (Cat # PAB14145) (0.1 ug/mL) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
CAMK2A polyclonal antibody (Cat # PAB14145) (3.8 ug/mL) staining of paraffin embedded human brain cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CAMK2A
Entrez GeneID
815Protein Accession#
NP_057065.2;NP_741960.1Gene Name
CAMK2A
Gene Alias
CAMKA, KIAA0968
Gene Description
calcium/calmodulin-dependent protein kinase II alpha
Omim ID
114078Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
Other Designations
CaM kinase II alpha subunit|CaM-kinase II alpha chain|CaMK-II alpha subunit|CaMKIINalpha|OTTHUMP00000165787|OTTHUMP00000165788|calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha|calcium/calmodulin-dependent protein kinase II alpha-B subunit
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Interactome
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Pathway
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Disease
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Publication Reference
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Tumor necrosis factor-alpha enhances neutrophil adhesiveness: induction of vascular cell adhesion molecule-1 via activation of Akt and CaM kinase II and modifications of histone acetyltransferase and histone deacetylase 4 in human tracheal smooth muscle cells.
Lee CW, Lin CC, Luo SF, Lee HC, Lee IT, Aird WC, Hwang TL, Yang CM.
Molecular Pharmacology 2008 May; 73(5):1454.
Application：IP, Human, Human tracheal smooth muscle cells.
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Tumor necrosis factor-alpha enhances neutrophil adhesiveness: induction of vascular cell adhesion molecule-1 via activation of Akt and CaM kinase II and modifications of histone acetyltransferase and histone deacetylase 4 in human tracheal smooth muscle cells.
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