AGAP2 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
AGAP2 polyclonal antibody (Cat # PAB14141) (0.3 ug/mL) staining of human amygdala lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of AGAP2.
Immunogen
A synthetic peptide corresponding to human AGAP2.
Sequence
C-QASLDSIREAVINSQ
Host
Goat
Theoretical MW (kDa)
90.5
Reactivity
Human
Specificity
Approx 100 KDa band observed in human brain (amygdala and cerebral cortex) lysates (calculated MW of 90.5 KDa according to NP_055585.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
AGAP2 polyclonal antibody (Cat # PAB14141) (0.3 ug/mL) staining of human amygdala lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — AGAP2
Entrez GeneID
116986Protein Accession#
NP_055585.1Gene Name
AGAP2
Gene Alias
CENTG1, FLJ16430, GGAP2, KIAA0167, PIKE
Gene Description
ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
Omim ID
605476Gene Ontology
HyperlinkGene Summary
O
Other Designations
Arf GAP with GTP-binding protein-like, ANK repeat and PH domains 2|GTP-binding and GTPase activating protein 2|centaurin, gamma 1|phosphoinositide 3-kinase enhancer
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Pathway
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Disease
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Publication Reference
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Neuroprotective actions of PIKE-L by inhibition of SET proteolytic degradation by asparagine endopeptidase.
Liu Z, Jang SW, Liu X, Cheng D, Peng J, Yepes M, Li XJ, Matthews S, Watts C, Asano M, Hara-Nishimura I, Luo HR, Ye K.
Molecular Cell 2008 Mar; 29(6):665.
Application：WB, Rat, Rat cortical neurons.
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Neuroprotective actions of PIKE-L by inhibition of SET proteolytic degradation by asparagine endopeptidase.
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