CD274 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of CD274 in Raji cell lysate with CD274 polyclonal antibody (Cat # PAB12985) at (A) 0.5 and (B) 1 ug/mL .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of CD274 in human heart tissue with CD274 polyclonal antibody (Cat # PAB12985) at 2.5 ug/mL .
Immunofluorescence
Immunofluorescence staining of human heart cells with CD274 polyclonal antibody (Cat# PAB12985) under 20 ug/mL working concentration.
Flow Cytometry
Flow cytometric analysis of A-20 cells with CD274 polyclonal antibody (Cat# PAB12985). Green: Isotype control. Red : CD274 antibody.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CD274.
Immunogen
A synthetic peptide corresponding to internal region 17 amino acids of human CD274.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
Has no cross-reactivity to CD273.
Form
Liquid
Purification
Peptide affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (0.5 ug/mL)
Immunofluorescence (20 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2.5 ug/mL)
Western Blot (0.5-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.02% sodium azide).
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of CD274 in Raji cell lysate with CD274 polyclonal antibody (Cat # PAB12985) at (A) 0.5 and (B) 1 ug/mL .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of CD274 in human heart tissue with CD274 polyclonal antibody (Cat # PAB12985) at 2.5 ug/mL .Immunofluorescence
Immunofluorescence staining of human heart cells with CD274 polyclonal antibody (Cat# PAB12985) under 20 ug/mL working concentration.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of A-20 cells with CD274 polyclonal antibody (Cat# PAB12985). Green: Isotype control. Red : CD274 antibody. -
Gene Info — CD274
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Interactome
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Pathway
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Disease
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Publication Reference
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Hepatic Artery Embolization Enhances Expression of Programmed Cell Death 1 Ligand 1 in an Orthotopic Rat Hepatocellular Carcinoma Model: In Vivo and in Vitro Experimentation.
Haruyuki Takaki, Yutaka Hirata, Eisuke Ueshima, Hiroshi Kodama, Seiji Matsumoto, Reona Wada, Hitomi Suzuki, Keiji Nakasho, Koichiro Yamakado.
Journal of Vascular and Interventional Radiology 2020 Sep; 31(9):1475.
Application：WB-Ce, Rat, RH7777 cells.
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Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1).
LaGier AJ, Pober JS.
Human Immunology 2006 May; 67(8):568.
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Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men.
Holling TM, Schooten E, van Den Elsen PJ.
Human Immunology 2004 Apr; 65(4):282.
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Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death.
Ishida Y, Agata Y, Shibahara K, Honjo T.
The EMBO Journal 1992 Nov; 11(11):3887.
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Hepatic Artery Embolization Enhances Expression of Programmed Cell Death 1 Ligand 1 in an Orthotopic Rat Hepatocellular Carcinoma Model: In Vivo and in Vitro Experimentation.
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