ACTA2 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Tissue lysate from mouse lung was resolved onto 12% SDS-PAGE and transferred onto NC Membrane, then probed by ACTA2 polyclonal antibody (Cat # PAB12649) at 1 : 500, right lane. Left lane was depleted by immunizing peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human colon tissue stained with ACTA2 polyclonal antibody (Cat # PAB12649) at 1 : 200 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ACTA2.
Immunogen
A synthetic peptide corresponding to 10 amino acids of N-terminus of human ACTA2.
Host
Rabbit
Theoretical MW (kDa)
42
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes ~42 KDa of ACTA2.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (2-5 ug/mL)
Flow cytometry (10-20 ug per 106 cells)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing. Upon initial thawing, apportion into working aliquots and store at -80°C for up to six months. -
Applications
Western Blot (Tissue lysate)
Tissue lysate from mouse lung was resolved onto 12% SDS-PAGE and transferred onto NC Membrane, then probed by ACTA2 polyclonal antibody (Cat # PAB12649) at 1 : 500, right lane. Left lane was depleted by immunizing peptide.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human colon tissue stained with ACTA2 polyclonal antibody (Cat # PAB12649) at 1 : 200 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
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Gene Info — ACTA2
Entrez GeneID
59Gene Name
ACTA2
Gene Alias
AAT6, ACTSA
Gene Description
actin, alpha 2, smooth muscle, aorta
Omim ID
102620Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
OTTHUMP00000020042|alpha 2 actin|alpha-cardiac actin|growth-inhibiting gene 46
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Interactome
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