CDKN1B polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot using CDKN1B polyclonal antibody (Cat # PAB10300) shows detection of CDKN1B protein in MCF-7 whole cell lysate (lanes 1-3) (arrowhead).
Separation was achieved using a 4-20% gradient gel.
Blocking occurred using 5% BLOTTO.
Primary antibody was diluted 1:500 in 1% BLOTTO.
The membrane was washed and reacted with a 1 : 10,000 dilution of Dylight™ 800 conjugated Gt-a-Rabbit IgG.
Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (Lane M).
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant CDKN1B.
Immunogen
Recombinant protein corresponding to full length human CDKN1B.
Host
Rabbit
Reactivity
Human
Specificity
Antiserum will specifically react with a p27 protein from human tissue. No reaction was observed against other related tumor suppressor proteins. No reactivity is observed with p27 from mouse.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
ELISA (1:10000-1:40000)
Western Blot (1:1000-1:4000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using CDKN1B polyclonal antibody (Cat # PAB10300) shows detection of CDKN1B protein in MCF-7 whole cell lysate (lanes 1-3) (arrowhead).
Separation was achieved using a 4-20% gradient gel.
Blocking occurred using 5% BLOTTO.
Primary antibody was diluted 1:500 in 1% BLOTTO.
The membrane was washed and reacted with a 1 : 10,000 dilution of Dylight™ 800 conjugated Gt-a-Rabbit IgG.
Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (Lane M).Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — CDKN1B
Entrez GeneID
1027Protein Accession#
P46527;NP_004055Gene Name
CDKN1B
Gene Alias
CDKN4, KIP1, MEN1B, MEN4, P27KIP1
Gene Description
cyclin-dependent kinase inhibitor 1B (p27, Kip1)
Gene Ontology
HyperlinkGene Summary
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. [provided by RefSeq
Other Designations
cyclin-dependent kinase inhibitor 1B
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Interactome
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Pathway
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Disease
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Publication Reference
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p130/pRb2 has growth suppressive properties similar to yet distinctive from those of retinoblastoma family members pRb and p107.
Claudio PP, Howard CM, Baldi A, De Luca A, Fu Y, Condorelli G, Sun Y, Colburn N, Calabretta B, Giordano A.
Cancer Research 1994 Nov; 54(21):5556.
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The retinoblastoma-related gene, RB2, maps to human chromosome 16q12 and rat chromosome 19.
Yeung RS, Bell DW, Testa JR, Mayol X, Baldi A, Grana X, Klinga-Levan K, Knudson AG, Giordano A.
Oncogene 1993 Dec; 8(12):3465.
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Cloning of a new member of the retinoblastoma gene family (pRb2) which binds to the E1A transforming domain.
Mayol X, Grana X, Baldi A, Sang N, Hu Q, Giordano A.
Oncogene 1993 Sep; 8(9):2561.
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p130/pRb2 has growth suppressive properties similar to yet distinctive from those of retinoblastoma family members pRb and p107.
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