Products

Western Blot (Cell lysate)

Immunoblot of HeLa cell extract.
All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature.
The membrane was blocked in 5% dry milk for 2 h.
After washing, a 1 : 1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h.
Washes with buffer were performed 4 times for 5' each.
The immunoblot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1 : 2,000 for 1 h.
Washes with TBS preceded color development.

Enzyme-linked Immunoabsorbent Assay

Entrez GeneID

4790

Gene Name

NFKB1

Gene Alias

DKFZp686C01211, EBP-1, KBF1, MGC54151, NF-kappa-B, NFKB-p105, NFKB-p50, p105, p50

Gene Description

nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

Omim ID

164011

Gene Ontology

Hyperlink

Gene Summary

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq

Other Designations

DNA binding factor KBF1|NF-kappabeta|nuclear factor NF-kappa-B p50 subunit|nuclear factor kappa-B DNA binding subunit|nuclear factor kappa-B, subunit 1