RB1 (phospho S811) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of RB1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S811 of human RB1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Dot Blot (1:500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with RB1 (phospho S811) polyclonal antibody (Cat # PAB0602) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.Dot Blot (Peptide)
Dot blot analysis of RB1 (phospho S811) polyclonal antibody (Cat # PAB0602) on nitrocellulose membrane. 50 ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. -
Gene Info — RB1
Entrez GeneID
5925Protein Accession#
NP_000312;P06400Gene Name
RB1
Gene Alias
OSRC, RB, p105-Rb, pRb, pp110
Gene Description
retinoblastoma 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq
Other Designations
OTTHUMP00000018397|retinoblastoma suspectibility protein|retinoblastoma-associated protein
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Interactome
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Pathway
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Disease
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Publication Reference
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Disruption of the Rb--Raf-1 interaction inhibits tumor growth and angiogenesis.
Dasgupta P, Sun J, Wang S, Fusaro G, Betts V, Padmanabhan J, Sebti SM, Chellappan SP.
Molecular and Cellular Biology 2004 Nov; 24(21):9527.
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ORF73 of herpesvirus saimiri, a viral homolog of Kaposi's sarcoma-associated herpesvirus, modulates the two cellular tumor suppressor proteins p53 and pRb.
Borah S, Verma SC, Robertson ES.
Journal of Virology 2004 Oct; 78(19):10336.
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Aberrant expression of pRb and p16(INK4), alone or in combination, indicates poor outcome after resection in patients with colorectal carcinoma.
Cui X, Shirai Y, Wakai T, Yokoyama N, Hirano S, Hatakeyama K.
Human Pathology 2004 Oct; 35(10):1189.
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Disruption of the Rb--Raf-1 interaction inhibits tumor growth and angiogenesis.
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