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Last updated: 2023/3/26

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Inorganic Pyrophosphatase (Yeast) BioActive

  • Catalog # : P8871
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  • Specification
  • Product Description:
  • Inorganic pyrophosphate (PPi) is generated as a reaction byproduct in many biosynthetic reactions which utilize ATP, including in vitro transcription and DNA polymerization. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate (P2O7-4 + H2O + PPase 2HPO4-2). PPase requires divalent metal cation (Mg2+) for its enzymatic activity.
  • Host:
  • Yeast
  • Form:
  • Liquid
  • Activity:
  • Standard reaction: 100 mM Tris-HCl (pH 7.2), 2 mM MgCl2 and 2 mM inorganic pyrophosphate.
  • Storage Buffer:
  • In 20 mM Tris-HCl, 100 mM KCl, pH 8.0 (0.1 mM EDTA, 1 mM DTT and 50% glycerol).
  • Storage Instruction:
  • Store at -20°C.
    Avoid repeated freezing and thawing.
  • Note:
  • Inorganic Pyrophosphatase (Yeast) requires divalent metal cation (Mg2+) for its enzymatic activity. This enzyme is widely used in RNA IVT reaction.
  • Applications
  • In vitro transcription
  • Functional Study
  • Application Image
  • In vitro transcription
  • Functional Study
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