The ED50 as determined by dose-dependent induction of IL-6 by mouse NIH 3T3 fibroblasts, is 0.44 - 0.66 ng/mL.
Storage Buffer:
Lyophilized from 10 mM sodium citrate, pH 3.0
Storage Instruction:
Lyophilized although stable at room temperature for 3 weeks. should be stored desiccated below -20°C. Upon reconstitution should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freezing and thawing.