ED50 < 10 ng/mL, measure by a cell proliferation assay using murine balb/c 3T3 cells, corresponding to a Specific Activity of 1 x 105Units/mg.
Surface Modification:
Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Storage Buffer:
Lyophilized from 20 mM Tris-HCl buffer(pH 8.0) cantaining?0.02% Tween-20 and 700 mM NaCl. Reconstitute the lyophilized powder in PBS to 100 ug/mL.
Storage Instruction:
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C. Avoid repeated freeze/thaw cycles.