ED50 < 25 ng/mL, determined by its ability to induce proliferation of mouse 3T3 cells in the presence of 0.1 ug/mL heprin, corresponding to a Specific Activity of > 4 x 104Units/mg.
Storage Buffer:
Lyophilized from a solution containing 1X PBS, pH 7.4. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction:
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze/thaw cycles.