ED50 < 20 ng/mL, determined by its ability to induce proliferation of mouse 3T3 cells, corresponding to a Specific Activity of 5 x 104Units/mg.
Storage Buffer:
Protein(1 mg/mL) was lyophilized from a solution containing 5mM Na3PO4, pH 7.5, 50 mM NaCl. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction:
Lyophilized protein at room temperature for 3 weeks, should be stored at -20°C. Protein aliquots at 4°C for 2-7 days and should be stored at -20°C to -80°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze/thaw cycles.