Human IL1A (P01583) partial recombinant protein expressed in HEK293 cells.
Host:
Mammals
Theoretical MW (kDa):
18
Form:
Lyophilized
Preparation Method:
Mammalian cell (HEK293) expression system
Purity:
> 97% by SDS-PAGE
Activity:
The ED50 is 0.5 - 5 ug/mL, measured by its binding ability in a functional ELISA. The ED50 is < 1 pg/mL, was determined by the dose-dependent stimulation of the proliferation of mouse D10S cells, corresponding to a specific activity of > 1x109 unit/mg.
Recommend Usage:
Biological Activity SDS-PAGE The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from sterile distilled Water up to 0.5 mg/mL
Storage Instruction:
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Aliquot to avoid repeated freezing and thawing.
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq