The ED50?is 5 - 25 ng/mL, calculated by the dose -dependent proliferation of human MCF/7 cells in the cell culture mixture dependent on culture conditions. ts activity consists of 30-40 % compared to human IGF-1.
Recommend Usage:
Biological Activity SDS-PAGE The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from sterile 0.4% NaHCO3, pH 8-9 > 100 ug/mL
Storage Instruction:
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Aliquot to avoid repeated freezing and thawing.