Mouse Il3 recombinant protein with polyhistidine tag at the C-terminus expressed in Escherichia coli.
Sequence:
MDTHRLTRTLNCSSIVKEIIGKLPEPELKTDDEGPSLRNKSFRRVNLSKFVESQGEVDPEDRYVIKSNLQKLNCCLPTSANDSALPGVFIRDLDDFRKKLRFYMVHLNDLETVLTSRPPQPASGSVSPNRGTVEC with polyhistidine tag at the C-terminus.
Host:
Escherichia coli
Form:
Lyophilized
Preparation Method:
Escherichia coli expression system
Purity:
≥ 95% as determined by SDS-PAGE.
Endotoxin Level:
< 0.1 EU per 1 ug of the protein by the LAL method.
Activity:
Measure by its ability to induce NFS-60 cells proliferation. The ED50 for this effect is < 85 pg/mL. The specific activity of recombinant mouse IL-3 is approximately > 1x 107 IU/mg.
Quality Control Testing:
SDS-PAGE Stained with Coomassie Blue.
SDS-PAGE analysis of Il3 (Mouse) Recombinant Protein.
Recommend Usage:
SDS-PAGE The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from a solution containing 1X PBS, pH 7.4. Reconstitute the lyophilized powder in ddH2O to 100 ug/mL.
Storage Instruction:
Lyophilized protein should be stored at -20°C. Protein aliquots should be stored at-20°C to -80°C. This product is stable for one year. Avoid repeated freeze/thaw cycles.