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Last updated: 2023/3/26

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Pkm (Mouse) Recombinant Protein BioActive

  • Catalog # : P8021
  • Visit Frequency :
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  • Specification
  • Product Description:
  • Mouse Pkm (P52480, 1 a.a. - 531 a.a.) full length recombinant protein with His tag expressed in Escherichia coli.
  • Sequence:
  • MPKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMNVARLNFSHGTHEYHAETIKNVREATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKEKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEAAAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDAVLNAWAEDVDLRVNLAMDVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP
  • Host:
  • Escherichia coli
  • Theoretical MW (kDa):
  • 60.2
  • Form:
  • Liquid
  • Preparation Method:
  • Escherichia coli expression system
  • Purity:
  • > 85% by SDS-PAGE
  • Activity:
  • Specific activity is > 50000 pmol/min/ug, in which one unit will convert 1.0 pmole of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37°C.
  • Quality Control Testing:
  • 3 ug by SDS-PAGE under reducing condition and visualized by Coomassie blue stain.

    QC Testing of P8021
  • Storage Buffer:
  • In 20mM Tris-HCl pH 8.0 (30% glycerol, 0.2 M NaCl, 1 mM DTT)
  • Storage Instruction:
  • Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Gene Name:
  • Pkm2
  • Gene Alias:
  • AA414905,AL024370,AL024424,Pk-2,Pk-3,Pk3
  • Gene Description:
  • pyruvate kinase, muscle
  • Other Designations:
  • pyruvate kinase 3
  • Interactome
  • Interactome
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