Specific activity is > 300 unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37°C.
Quality Control Testing:
3 ug by SDS-PAGE under reducing condition and visualized by Coomassie blue stain.
Storage Buffer:
In 20mM Tris-HCl pH 8.0 (40% glycerol, 1 mM EDTA, 1 mM DTT, 0.1 mM PMSF)
Storage Instruction:
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Aliquot to avoid repeated freezing and thawing.
DUSP18 is a member of the dual-specificity phosphatase (DSP) family (see DUSP1; MIM 600714), which catalyzes dephosphorylation of phosphotyrosine and phosphothreonine residues (Hood et al., 2002 [PubMed 12408986]).[supplied by OMIM