Specific activity is > 1300 nmol/min/ug, and is defined as the amount of enzyme that cleaves 1 nmol of suc-AAPF-pNA per minute at 37°C in Tris-HCl pH 8.0 using chymotrypsin.
Quality Control Testing:
3 ug by SDS-PAGE under reducing condition and visualized by Coomassie blue stain.
Storage Buffer:
In Phosphate-Buffer Saline pH 7.4 (10% glycerol, 1 mM DTT)
Storage Instruction:
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Aliquot to avoid repeated freezing and thawing.