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Last updated: 2023/3/26

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RNASE1 (Human) Recombinant Protein BioActive

  • Catalog # : P7881
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  • Specification
  • Product Description:
  • Human RNASE1 (P07998, 29 a.a. - 156 a.a.) partial recombinant protein with His tag expressed in HEK293 cells.
  • Sequence:
  • KESRAKKFQRQHMDSDSSPSSSSTYCNQMMRRRNMTQGRCKPVNTFVHEPLVDVQNVCFQEKVTCKNGQGNCYKSNSSMHITDCRLTNGSRYPNCAYRTSPKERHIIVACEGSPYVPVHFDASVEDST
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 15.3
  • Form:
  • Liquid
  • Preparation Method:
  • Mammalian cell (HEK 293) expression system
  • Purity:
  • > 90% as analyzed by SDS-PAGE.
  • Endotoxin Level:
  • < 1 EU/ug of protein by the LAL method.
  • Activity:
  • Specific activity is > 3 x 106 unit/mg, and is defined as the amount of enzyme that cleaves 1.0 pmole of RNase probe per minute at 25°C.
  • Quality Control Testing:
  • SDS-PAGE Stained with Coomassie Blue.

    QC Testing of P7881
    SDS-PAGE analysis of RNASE1 (Human) Recombinant Protein.
  • Recommend Usage:
  • Biological Activity
    SDS-PAGE
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 ( 10% glycerol)
  • Storage Instruction:
  • Store at 4°C for 1 week. For long term storage store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Functional Study
  • SDS-PAGE
  • Application Image
  • Functional Study
  • SDS-PAGE
  • Gene Information
  • Entrez GeneID:
  • 6035
  • Gene Name:
  • RNASE1
  • Gene Alias:
  • MGC12408,RIB1,RNS1
  • Gene Description:
  • ribonuclease, RNase A family, 1 (pancreatic)
  • Gene Summary:
  • This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq
  • Other Designations:
  • RNase upI-1,pancreatic ribonuclease
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