< 0.2 EU per 1 ug of protein (determined by gel clotting method)
Activity:
The ED50 was determined by a cell proliferation assay using Balb/c 3T3 cells is < 0.2 ng/mL, corresponding to a specific activity of > 5.0 × 106 units/mg.
Storage Buffer:
Lyophilized from sterile distilled Water up to 100 ug/mL
Storage Instruction:
Store at 4°C to 8°C for 1 week. For long term storage store at -20°C to -80°C. Aliquot to avoid repeated freezing and thawing.
Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. [provided by RefSeq